cellometer auto t4 user manual

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cellometer auto t4 user manual

Our lab is crowded and the compact size allowed us to squeeze it into the small amount of spare space we have. This counter came highly recommended from a colleague and so we had to get one for ourselves. It has made counting cells far more accurate and easier for us compared to our old counters and shared equipment. Our lab is crowded and the compact size allowed us to squeeze it into the small amount of spare space we have. It has made counting cells far more accurate and easier for us compared to our old counters and shared equipment. However, creating settings for a new cell type is very easy. Overall, its reliable and a time-saving machine. There is a large library of settings for different type of cells. However, creating settings for a new cell type is very easy. Overall, its reliable and a time-saving machine. Cell counting has been made easier through the Cellometer Auto T4 instrument. The software is easy to access and user-friendly. Handling of the instrument is also user-friendly. Cell counting has been made easier through the Cellometer Auto T4 instrument. Handling of the instrument is also user-friendly. It looks like a simple instrument, yet provides complete information of the cell counting: viable cells, dead cells, viability, total cell numbers. It also provides cell sizes and distribution that are very informative to help you know your cells better. This instrument is already trained for many commonly used cells in the lab. Great. It looks like a simple instrument, yet provides complete information of the cell counting: viable cells, dead cells, viability, total cell numbers. This instrument is already trained for many commonly used cells in the lab. Great. Now I don’t have to manually count the cell number on a neubauer chamber under the microscope and stress my eyes. Cellometer is very easy to use, fast and accurate.

The best thing is that I can optimize the settings for counting different cells on the basis of size and it can even count clumpy cell lines. I would definitely recommend this instrument to everyone. Now I don’t have to manually count the cell number on a neubauer chamber under the microscope and stress my eyes. I would definitely recommend this instrument to everyone. I save a lot of time when I count number of cells and more when I am using more than one cell line. I save a lot of time when I count number of cells and more when I am using more than one cell line. We recommend not only these machines, but this company also. The customer service is good too, willing to come and help set the optimization of the instrument. We recommend not only these machines, but this company also. I like the capability of the counter to show you which cells were counted. It is usually very accurate in picking up cells, including detecting individual cells in clusters and excluding all junk. It has some unique features not avail on the other counters. I like the capability of the counter to show you which cells were counted. It is usually very accurate in picking up cells, including detecting individual cells in clusters and excluding all junk. It gets better if you have the autofocus function. We are satisfied with the function. It gets better if you have the autofocus function. I love it! It speeds up my work significantly. I love it! Jul 19, 2018 We like it very much! We like it very much! Cell count, concentration, diameter, and % viability are automatically calculated and reported. The dye is unable to penetrate healthy cells, so they remain unstained. Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells are stained blue and display as dark cells in the Cellometer software with bright field imaging.

Isolated from various tissues (bone marrow, lung, ovary, brain, skin, breast, colon, kidney, prostate), the cell lines are used extensively to screen experimental cancer drug compounds and study tumor development. The Cellometer pattern-recognition software identifies and counts individual cells within these cell clumps for accurate analysis (shown at left). With the Auto T4 Cell Counter, cell morphology can be immediately viewed on-screen. This is equivalent to the area of four quadrants of a hemocytometer. Counted cells are indicated on-screen for further verification that cells in the sample are being imaged and analyzed properly. Counting live and dead cells sometimes takes twice as long. The Cellometer Auto T4 Cell Counter calculates cell count and concentration for live and dead cells and % viability in just 10 seconds. The CVs for manual counts ranged from 7% to 20%. Automated cell counters remove inter-operator variability and subjectivity from cell counts to generate more precise data. Images and data tables can be pasted into PowerPoint presentations or submitted for publication. View sample data file. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. View sample report. Cell suspension of 20 microliters is loaded into the chamber using a standard single channel pipette. The chamber is inserted into the Cellometer cell counter and the cells are imaged. This simple sample loading and analysis method is ideal for fragile cells. Please inquire for pricing. Multiple viability stains, such as trypan blue, propidium iodide are used to identify dead cells.For adherent cell lines, cell characteristics are based on the appearance of cells following trypsinization. Our lab is crowded and the compact size allowed us to squeeze it into the small amount of spare space we have. Overall, its reliable and a time-saving machine. However, creating settings for a new cell type is very easy.

I would definitely recommend this instrument to everyone. I save a lot of time when I count number of cells and more when I am using more than one cell line. I save a lot of time when I count number of cells and more when I am using more than one cell line. We recommend not only these machines, but this company also. We recommend not only these machines, but this company also. I like the capability of the counter to show you which cells were counted. It is usually very accurate in picking up cells, including detecting individual cells in clusters and excluding all junk. It gets better if you have the autofocus function. It gets better if you have the autofocus function. I love it! It speeds up my work significantly. I love it! We like it very much! We like it very much! Nexcelom products are for RESEARCH USE ONLY and are not approved for diagnostic or therapeutic use. Building 9, Lawrence, MA, 01843 Search for more papers by this author Published Online: 16 May 2018 About Sections View PDF Counting results contribute significantly to assay accuracy. However, currently available automatic cell counting methods often require costly instruments with liquid handling and cleaning hardware. Daily usage requires special reagent and sample preparation, along with routine calibration and maintenance. Due to these limitations, cell counting remains largely a traditional manual operation with hemacytomers for most research laboratories. Like using a hemacytometer, it requires no special counting buffer or cell manipulation. Cells in growth media, PBS or other solutions can be pipetted directly into disposable counting chambers. For cell viability, the standard trypan blue method is used. By using disposable, all plastic counting chambers, samples are isolated within each counting chamber, thus eliminating needs for liquid handling hardware and risk of cross contamination by different samples. There is no washing between each sample and no chance to clog the system.

The system includes a compact instrument with advanced imaging-based cell counting software, which automatically analyzes acquired cell images and measures cell concentration, viability, and cell size. Each type of cell uses a set of cell type parameters, which are established and saved for repeated use. For a new cell type, parameters can be imported from a library of cell type parameters, or they can be established automatically using a “New Cell Type Wizard”. Once set up, switching from one cell type to another only involves the selection from a drop down menu. For attachment cells, they were trypsinized prior to loading into a counting chamber. For cell suspension diluted prior to loading into counting chamber, a dilution factor was applied for the software to directly convert cell concentration results to reflect the dilution. Results and Conclusions Some representative data are shown in Figures 1 and 2. Cells were suspended in respective growth media or PBS. For high viability culture, total cell counts were used. To obtain viable cell concentration, treat cells with trypan blue. The option of total cell count or viable cell count is selected prior to counting. When viability option is selected, cell concentration, viability data were produced in a single sample within the same process. Figures References Related Details. By using advanced cell counting chamber technology, Cellometer Auto T4 eliminates cross contamination, allows for no maintenance and immediate setup and use. This makes it suitable for occasional use or users with a larger volume of cell samples. Hemacytometer PBMC Dilution Series L929 Dilution series Cellometer Auto T4: Automates manual cell counting in less than 30 seconds. It is also an essential step in seeding cells for downstream experiments, with accurate counting critical to ensuring experimental reproducibility.

Although researchers have for years relied on traditional glass hemocytometers to generate cell counts, there has been a steadily growing trend toward the use of alternative methods. These aim to eliminate user-to-user variance, speed up the counting process, and deliver more reliable results. Stuart Wood, research scientist at Bactevo, which is developing drugs to treat diseases that involve defects in mitochondrial function, routinely performs assays using HepG2, HeLa, HEK293, and other standard cell lines, in addition to iPSCs. He currently uses disposable hemocytometers to count his cells. “Disposable systems provide several advantages over glass hemocytometers,” explains Wood. “Not only can glass hemocytometers be subject to contamination, but it is also possible for the cover slide to lift slightly if greater than the recommended volume of liquid is introduced to the counting chamber. This can result in counting inaccuracies, which will impact on experimental outcomes. Glass hemocytometers can also be easily damaged.” Although Wood does not routinely count cells for passaging, he explains that “counting helps those who are newcomers to cell culture learn how to split cells at an appropriate time and at the correct ratio. It’s also extremely useful for more temperamental cell lines, which perhaps can’t tolerate being split too heavily. Furthermore, counting has huge utility when you wish to accomplish multiple transfections with different cell lines simultaneously since it allows you to ensure that all the cells are ready at the same time.” Wood is a strong advocate of automated cell counters. “Automated systems often provide a live:dead ratio. This can flag up any issues, and is particularly useful when you’re generating a new cell line or performing a transfection as it provides an indication of whether the modification you have made is toxic.” Automated instrumentation Beckman Coulter is a name synonymous with cell counting.

According to Stephen Munt, cell analysis specialist at Beckman Coulter, the company’s current flagship Coulter Counter is the MULTISIZER 4e. We developed a disposable hemocytometer, the CP2, with an all-in-one design to eradicate the need for cleaning or assembly of parts, yet although this was hugely popular it still required that a manual cell count be performed. Extensive customer feedback informed us that not only did researchers not want the hassle of preparing a hemocytometer, but they would also prefer not to spend time at the microscope performing multiple cell counts by eye. As a result we invented our Cellometer Auto T4, a chip-based instrument for measuring cell concentration and viability.” The use of a slide avoids the need for a liquid-handling system, meaning that there is no need for a regular cleaning routine to be implemented, while the included software enhances workflow efficiency by performing calculations for the end user. Following the success of the Cellometer Auto T4, Nexcelom expanded its product range to include instrumentation for more advanced cellular analysis. “All cells are different,” explains Qiu, “and choosing an appropriate cell counter is not a trivial matter. Although any cell counter can produce a numerical readout, we believe that a truly accurate cell count is purpose dependent, and we’ve designed our next-generation systems with this in mind.” Tiffany Sidwell, clinical laboratory scientist apprentice at UCLA molecular diagnostics laboratories, is a regular user of Nexcelom’s Auto 2000 instrument, which is specifically optimized for analysis of primary cells from complex heterogeneous samples such as peripheral blood, cord blood, or bone marrow. The instrument uses dual-color fluorescence for identifying both live and dead stained nucleated cells, enabling the generation of accurate viability results even in the presence of debris, platelets, and red blood cells.

“We routinely count PBMCs and cryopreserve them at a specific number per vial,” says Sidwell, “and since adding the Auto 2000 to the lab, our samples have become more reliable in terms of viability and accuracy of counts.” Image: Due to hepatocytes' variable morphology, fragile nature, and tendency to clump, traditional manual counting methods can be time-consuming and inaccurate. Cellometer K2 or Cellometer Vision capture and automatically count bright field and fluorescent images. While the bright field image shows cell morphology the fluorescent image shows live cells stained with acridine orange (green) and dead cells stained with propidium iodide (red). Image courtesy of Nexcelom Biosciences. Data analysis can be performed on the device, or alternatively results can be uploaded to a computer. High-resolution measurements of cell size and volume are used to generate a histogram distribution. “Gating allows you to focus on your population of interest, while quantitative data on cell populations can be used to evaluate the quality and health of the culture,” Tiano adds. While manual counting has not yet fallen from favor, companies such as Beckman Coulter, Nexcelom Bioscience, and MilliporeSigma are doing all they can to provide researchers with affordable measures to improve the efficiency and accuracy of their cell counts. Whether a disposable hemocytometer, an automated benchtop system, or a handheld device is chosen, these modern systems supply huge benefits and their utility is flourishing. It uses brightfield microscopy images to analyze cell samples to determine cell concentration and trypan blue viability. Residual red blood cell concentrations do not influence PBMC concentration and viability measurement.

Easy It’s quick and simple to do Fast Your inquiry will be delivered straight to the manufacturer Free You’re under no obligation Secure We only pass your details on to trusted suppliers at your request Save time Submit your details once and make multiple inquiries. Advanced image analysis software provides one-step, size-based concentration and viability measurements using clumpy or irregularly shaped cells, excluding debris, with data in less than 60 seconds. Cell count, concentration, diameter, Acridine orange (AO) It stains all nucleated cells to There is no need to lyse red blood cells, saving time and The dye is unable to penetrate Dead cells have a compromised cell membrane that is permeable to the trypan blue dye. Dead cells It stains all dead nucleated cells to generate red fluorescence. Cells appearing in both the bright field and fluorescent image are dead.The Cellometer Auto X4 (10x) is ideal for Counted bright field images show counted cells Analysis of The Cellometer pattern-recognition software identifies Cells can be stained Cells can be stained Images and data tables can be pasted into PowerPoint presentations or submitted for publication. The standard printout displays live, dead, and total cell count and concentration, % viability, and cell images. Cell suspension of 20 microliters is loaded into the chamber Please inquire for pricing. It is the best thing in the lab since pipettman. - Carolyn Cooke, MD AndersonThe cell count is more accurate than Trypan Blue and gives a count for live and dead cells. It has really helped increase consistency in my experiments! The AOPI solution makes this process a breeze. Furthermore, the technical support we have gotten from our Nexcelom representative is outstanding. We also love the fact that the K2 software upgrades are free. Overall, our experience with the K2 instrument and Nexcelom is excellent. Also, what is considered a live cell vs.

The counting is consistent, the purity check that it does for us is amazing.This is critical for our downstream analysis. The results are both accurate and reproducible. We use it every single day in our lab! A tedious job has been eliminated. Thanks Nexcelom! - John Sipes, NCI We love to use it for our single cell sequencing platform. We found that counting yeast cell cultures is very fast and accurate, especially since we can change the parameters. For example, we can change cell counts based on cell size. Also, there is an option of declustering which is exceptionally helpful for yeast cultures that are arrested at the G2 stage of the cell cycle, which is necessary for many of our experiments. - Cynthia Sakofsky, NIEHS He was also quick to send me a quote and arrange the install and set up of the instrument. He went over all the steps with me and made sure everything was okay. When I attempted to use the instrument alone for the first time, I had some initial trouble with USB connectivity. I contacted technical support and they found a solution in less than 1 hour and emailed me. Now the machine is running smoothly and I am happy with it! Being able to program different cell types is also a great feature.? - Colleen Worne, University of Utah We already have more than four K2 cell counting instruments. This is our preferred instrument to use by far! The cell counts are fast, consistent and reliable. The T4 software is very user-friendly! It is designed for simple, accurate analysis of nucleated cells, including PBMCS, splenocytes, and stem cells, in peripheral blood, cord blood, bone marrow and other heterogeneous samples. - Ling Li, City of Hope It has saved a lot of time from counting cells with hemacytometer. It doesn't take much space either! - Jiayuan Shinkle, The Jackson Laboratory When I moved to a different company about 6 months ago, I bought a Cellometer Auto 2000 for my lab and my lab mates love it.

I work mostly on human samples and counting using the Cellometer with AOPI is way more accurate than the Vi-Cell or any other counting instrument I've used! - Candy Garcia, Ideaya Bioscience It makes it very simple to count primary cells. I have used this counter over the last 7 years, so I can't imagine any other counters replacing this! We love that the dye is nuclear to ensure accuracy of the cell counts. Sure beats counting by hand!? - Amanda Clark, Biomere When doing cell culture of primary cells it is important to know what you're dealing with in a timely manner so you can plate the appropriate cell density. The Auto 2000 is a good tool for making this process fast, efficient and accurate. It is a vast improvement over using Trypan Blue and gives results that we can trust! You can see pictures of your cells and cell counts within seconds. It makes me confident in my cell counts when I am preparing an experiment. In addition, the system is very amenable to electronic documentation of counts.? - Toai Nguyen, Davinci Biosciences, LLC I culture a lot of cell lines at one time so cell counting is time-consuming. This cell counter helps me a lot! It looks very nice and is not very thick for handling. The two-sided film slides are my favorite, because I don’t need to worry about the contamination of hands or my bench! Excellent technical support to help write project specific programs. - Casey Bucan, Waisman Biomanufacturing It saves a lot of time and is very convenient for counting cells, requiring very little sample. It doesn't take up much room either!? - Laura Garcia, UPMC Everyone in my lab loves to use it. We count everything from human cells to insect cells. No more painstaking hand counting using the hemacytometer. Thanks so much Nexcelom! - Solomon C. Amadiume, Weill Cornell Medical College I am now able to count many cell lines within just a few minutes!? - Anita Robin, Weill Cornell Medical College It makes cell counting a breeze.

It is quick and very easy to use. You can count your cells with a turn of a knob and with a click of a button! Out lab processes PBMCs, counts them and then places them into a 2 week in-vitro stimulation culture in the presence of peptide pools. Two weeks later we harvest the cells, count them and then run them in both an Elispot and a Cytokine stimulation assays. The use of the Auto 2000 cell counter has made the counting of cells much easier in much less time.? - Natalia Jun, Human Longevity, Inc. It gives us viability and recovery is easy to calculate. It is very user friendly and we can always save the data on our computer once the counting is done! - Mukesh Mudgal, Florida International University Our local Nexcelom representative was amazing and made the transition process extremely simple! Earlier, I used to work with the Cellometer Auto T4 but then switched to the Vision CBA. With the Cellometer, I consumed less time and it helps me get research work done effectively! Everyone in the lab loves it. It is a great product overall! I use it nearly daily to count hepatocytes and it is practically effortless. What a great consistent counting instrument. And with knowing the accurate cell count, I can give more of a precise count of levels of my target cell type. We are pleasantly surprised to find the accompanying laptop already loaded with the necessary software upon purchasing it. Nexcelom customer service has been excellent as well! It is very easy to use and train others on! You can tell right away how your cell density is and if the cell suspension needs to be diluted. And it uses far less sample than the Nucleocounter without needing to dilute sample! It provides improved counting accuracy and viability information on cells counted and also gives us the ability to refer back to previously counted cell samples as needed. It has been great for my lab group, we would be lost without it! It has saved us time and space, especially during lengthy experiments.

We used it for nucleated cell count, stained with AO. The instrument is very user friendly, as well as the platform - you can adjust the focus and exclude stuff you don’t want to have counted. I've been using the same instrument for over 2 years now and never have any problem with it. We perform QC every day, and is always within range. The Cellometer is a very good and easy going piece of equipment. I trust that they give us comparable results, which is invaluable for ensuring consistency of our fermentations and products across locations! It counted my cells quickly and accurately, which is vital for my cell culture talks required of me weekly! Only 20 uL of cells are needed for each count, which is essential for some precious samples like primary cells. Now it is very easy for me to set up experiments with different cell lines. Getting accurate cell count is so easy with the Auto T4. - Leticia Reyes Ramos, Medical University of South Carolina - Pharmacology Also, the unit is reliable and needs little to no maintenance. It just always works well! - Sarah DeLorenze, Two Roads Brewing Company It allows counting of multiple cell samples and is easy to use for a lot of different cell types. It is superior for determining cell viability of immune cells. They are very easy to use and count the cells very fast! It always gives us great reproducibility between cell counts and has increased our throughput drastically. Not only is it much faster than using the hemacytometer, it is much more reproducible between operators. Good quality cell counter. Our results are very reproducible and reliable! - Roshan Kumari, UTHSC It gives clear images quickly. We have been using it for a couple years and never have any problems with it! - Solaema Taleb, IUPUI Both cell counters have saved us critical time and are very easy to use. The Cellometer Auto 2000 has been invaluable at helping up with difficult-to-count cells, and even with developing new assays.

Both are indispensable pieces of equipment in our lab. After trying our Auto 2000, another lab in our building is purchasing one for their own use. I can get through my experiments much quicker, especially when I have numerous samples. It is also helpful when I have a very high cell concentration because I can trust that I am getting an accurate cell count without a lot of effort on my part. It also makes cell culturing experiences much quicker and less painful than previously. It is much easier and more efficient than doing it by hand. As a new student in the lab, I am grateful to have cool technology to help me out! There is no lag when adjusting the mirror's focus as well. Cell counts are done precisely and in a timely manner. - Andres Enrique Martinez-Muniz, University of Puerto Rico Mayaguez Nexcelom employees are very kind and helpful, the service was very fast and easy to order from, as well as the technical help in using the instrument! - Tina Yaskell, Forsyth Institute It gives very precise results in a very short time, and only uses a small amount of sample, which makes it very efficient for research purposes. - Nelson Franqui, Darlington Building It counts cells quickly and saves me a lot of time! Our Cellometers are reliable and every time I call for a products or technical service, I have spoken to a helpful and knowledgeable person. It allows us to count cells to determine how many to use for our flow experiments. I would recommend it! - Deepak Kanojia, Kanojia It is easy to use and very accurate in cell counting. Using this cell counter give more homogeneous results with different lab individuals. In addition, it has a vast range for counting cells so you do not need to dilute your sample again to be in the range of the cell counter. - Rohit Upadhyay, Tulane University We like it so much we got spoiled and bought another one. No waiting to count. Everyone is happy and loves this machine!

Both instruments have made cell counting amazingly simple and accurate for live dead cells. We appreciate the training! We are also using it to count beads that are 50um in diameter and the numbers are extremely accurate. Our local Applications Scientist from Nexcelom was very thorough for the training process and really helpful in establishing relevant protocols. Nexcelom's Cellometer Mini improves cell counting accuracy and saves our scientists a lot of time! - Anita Tang, iXCells Biotechnologies We love this machine. It always gives us quick and clear cell counts! It is very easy to use and the programs are very user friendly. Plating cells is a super quick process now! - Connor Dyer, Stanley Manne Children's Research Institute Just choose programs for your cells and focus. It is much faster and accurate than hemacytometer! It works like a champ. It saves me so much time. Our entire department loves this Cellometer! I recommend it for anyone looking to ditch the hemacytometer! It has been a pleasure working with Nexcelom's group. All questions have been answered in a timely fashion and customer service has been excellent! - Martin Lagrange, Wuci AppTec I enjoyed looking at the images to see immune cell killing. Working with our representative from Nexcelom was great. He was incredibly helpful and available at all times in the day! The time in the TC room has gone way down, and cell viability is always where I want it! I've never had any issues with the machine malfunctioning or the software crashing. It is also very easy to import datasets from the software, making it easy to extract and analyze data. - Samantha Stanley, University of Houston It's so fast and convenient compared to the traditional way of counting. My favorite feature is that you can manually change the cell count numbers slightly if needed. Live and dead cells can be distinguished very clearly. We can also look at the past pictures of the cells and their counts!